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   Ethyl Caffeate Ameliorates Collagen-Induced Arthritis by Suppressing Th1 Immune Response  
   
نویسنده xu s. ,zuo a. ,guo z. ,wan c.
منبع journal of immunology research - 2017 - دوره : 2017 - شماره : 0
چکیده    The present study was designed to assess the antiarthritic potential of ecf in collagen-induced arthritis (cia) and explore its underlying mechanism. methods. in vitro,lymphocyte proliferation assay was measured by cck-8 kit. in vivo,the therapeutic potential of ecf on cia was investigated; surface marker,treg cell,and intracellular cytokines (il-17a and ifn-γ) were detected by flow cytometry. th1 cell differentiation assay was performed,and mrna expression in interferon-γ-related signaling was examined by q-pcr analysis. results. in vitro,ecf markedly inhibited the proliferation of splenocytes in response to cona and anti-cd3. in vivo,ecf treatment reduced the severity of cia,inhibited ifn-γ and il-6 secretion,and decreased the proportion of cd11b+gr-1+ splenic neutrophil. meanwhile,ecf treatment significantly inhibited the ifn-γ expression in cd4+t cell without obviously influencing the development of th17 cells and t regulatory cells. in vitro,ecf suppressed the differentiation of naive cd4+ t cells into th1. furthermore,ecf intensely blocked the transcriptional expression in interferon-γ-related signaling,including ifn-γ,t-bet,stat1,and stat4. conclusion. our results indicated that ecf exerted antiarthritic potential in collagen-induced arthritis by suppressing th1 immune response and interferon-γ-related signaling. © 2017 shikui xu et al.
آدرس school of pharmacy,xi'an jiaotong university,xi'an,china,yunnan institute for food and drug control,kunming, China, school of pharmacy,yunnan university of traditional chinese medicine,kunming, China, school of pharmacy,xi'an jiaotong university,xi'an, China, central laboratory,no.1 affiliated hospital of yunnan university of traditional chinese medicine,kunming, China
 
     
   
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