Role of CD8 Regulatory T Cells versus Tc1 and Tc17 Cells in the Development of Human Graft-versus-Host Disease

Cd8+ t cells that secrete proinflammatory cytokines play a central role in exacerbation of inflammation; however,a new subpopulation of cd8 regulatory t cells has recently been characterized. this study analyzes the prominent role of these different subpopulations in the development of graft-versus-host disease (gvhd). samples from 8 healthy donors mobilized with filgrastim� (g-csf) and 18 patients who underwent allogeneic hematopoietic stem cell transplantation (hsct) were evaluated by flow cytometry. mobilization induced an increase in tc1 (p < 0.01),th1 (p < 0.001),tc17 (p < 0.05),and cd8+il-10+ cells (p < 0.05),showing that g-csf induces both pro-and anti-inflammatory profiles. donor-patient correlation revealed a trend (p = 0.06) toward the development of gvhd in patients who receive a high percentage of tc1 cells. patients with acute gvhd (agvhd),either active or controlled,and patients without gvhd were evaluated; patients with active agvhd had a higher percentage of tc1 (p < 0.01) and tc17 (p < 0.05) cells,as opposed to patients without gvhd in whom a higher percentage of cd8 treg cells (p < 0.01) was found. these findings indicate that the increase in tc1 and tc17 cells is associated with gvhd development,while regulatory cd8 t cells might have a protective role in this disease. these tests can be used to monitor and control gvhd. � 2017 adriana guti�rrez-hoya et al.