Nutrient induced type 2 and chemical induced type 1 experimental diabetes differently modulate gastric GLP-1 receptor expression

T2dm patients demonstrate reduced glp-1 receptor (glp-1r) expression in their gastric glands. whether induced t2dm and t1dm differently affect the gastric glp-1r expression is not known. this study assessed extrapancreatic glp-1r system in glandular stomach of rodents with different types of experimental diabetes. t2dm and t1dm were induced in psammomys obesus (po) by high-energy (he) diet and by streptozotocin (stz) in sprague dawly (sd) rats,respectively. glp-1r expression was determined in glandular stomach by rt pcr and immunohistomorphological analysis. the mrna expression and cellular association of the glp-1r in principal glands were similar in control po and sd rats. however,nutrient and chemical induced diabetes resulted in opposite alterations of glandular glp-1r expression. diabetic po demonstrated increased glp-1r mrna expression,intensity of cellular glp-1r immunostaining,and frequency of glp-1r positive cells in the neck area of principal glands compared with controls. in contrast,sd diabetic rats demonstrated decreased glp-1 mrna,cellular glp-1r immunoreactivity,and frequency of glp-1r immunoreactive cells in the neck area compared with controls. in conclusion,nutrient and chemical induced experimental diabetes result in distinct opposite alterations of glp-1r expression in glandular stomach. these results suggest that induced t1dm and t2dm may differently modulate glp-1r system in enteropancreatic axis. copyright © 2015 olga bloch et al.