Association of a large panel of cytokine gene polymorphisms with complications and comorbidities in type 2 diabetes patients

Aims. the polymorphisms of pro-and anti-inflammatory cytokines may be involved in type 2 diabetes (t2d) pathogenesis and its complications. methods. we investigated in 102 t2d patients the association of the cytokine polymorphisms in the tnf-α,il-10,il-6,tgf-β1,and ifn-γ genes with the t2d microvascular complications and comorbidities (hypertension,dyslipidemia,and obesity). cytokine genotypes were determined by pcr using cytokine genotyping tray kit. results. diabetic retinopathy was associated with gg genotype and g allele in tgf-β1 codon 25c/g polymorphism (p=0.004 and p=0.018) and the nephropathy was associated the lower frequency of gg genotype in il-10-1082g/a polymorphism (p=0.049). hypertension was associated with the cc genotype and c allele for il-10-592c/a polymorphism (p=0.013 and p=0.009) and higher frequencies of t (p=0.047) and c (p=0.033) alleles of the tgf-β1 codon 10t/c and il-10-819t/c polymorphisms,respectively. the tgf-β1 codon 10t/c polymorphism was associated with the bmi groups (p=0.026): the cc genotype was more frequent in the group with bmi < 25 kg/m2,while the tc genotype was more frequent in the group with bmi = 30 kg/m2. conclusions. our findings suggest that tgf-β1 and il-10 polymorphisms are involved in complications and comorbidities in t2d patients. © 2015 k. f. rodrigues et al.