Effect of Human Myotubes-Derived Media on Glucose-Stimulated Insulin Secretion

Fasting to postprandial transition requires a tight adjustment of insulin secretion to its demand,so tissue (e.g.,skeletal muscle) glucose supply is assured while hypo-/hyperglycemia are prevented. high muscle glucose disposal after meals is pivotal for adapting to increased glycemia and might drive insulin secretion through muscle-released factors (e.g.,myokines). we hypothesized that insulin influences myokine secretion and then increases glucose-stimulated insulin secretion (gsis). in conditioned media from human myotubes incubated with/without insulin (100 nmol/l) for 24 h,myokines were qualitatively and quantitatively characterized using an antibody-based array and elisa-based technology,respectively. c57bl6/j mice islets and wistar rat beta cells were incubated for 24 h with control and conditioned media from noninsulin- and insulin-treated myotubes prior to gsis determination. conditioned media from insulin-treated versus nontreated myotubes had higher rantes but lower il6,il8,and mcp1 concentration. qualitative analyses revealed that conditioned media from noninsulin- and insulin-treated myotubes expressed 32 and 23 out of 80 myokines,respectively. islets incubated with conditioned media from noninsulin-treated myotubes had higher gsis versus control islets (p<0.05). meanwhile,conditioned media from insulin-treated myotubes did not influence gsis. in beta cells,gsis was similar across conditions. in conclusion,factors being present in noninsulin-stimulated muscle cell-derived media appear to influence gsis in mice islets. © 2017 maria l. mizgier et al.