Development and validation of a RP-HPLC method for determination of nimodipine in sustained release tablets

A rapid,sensitive,and reproducible reverse phase high performance liquid chromatographic (rp-hplc) method with uv detector for the determination of nimodipine in sustained release tablets was developed. the method involved using a sinochoom ods-bp c18 reversed phase column (5 m,4.6 mm × 200 mm) and mobile phase consisting of methanol-acetonitrile-water (35: 38: 27,v/v). the flow rate is 1.0 ml/min,the uv detector was operated at 237 nm,and the column was maintained at 25°c. the method was validated according to official compendia guidelines. the calibration curve of nimodipine for rp-hplc method was linear over the range of 10-100 g/ml. the retention time was found at 7.50 min for nimodipine. the variation for interday and intraday assay was found to be less than 0.72%. the proposed rp-hplc was proved to be suitable for the determination of nimodipine in sustained release tablets. © 2013 xiaojun shang et al.