Optimization of synthesis of seleno- sargassum fusiforme (Harv.) Setch. polysaccharide by response surface methodology,its characterization,and antioxidant activity

The response surface methodology was employed to optimize the synthesis conditions of seleno-sargassum fusiforme (harv.) setch. polysaccharide. three independent variables (reaction time,reaction temperature,and ratio of na 2seo3 to sfpsi) were tested. furthermore,the characterization and antioxidant activity of se-sfpsi in vivo were investigated. the result showed that the actual experimental se content of se-sfpsi was 3.352 mg/g at the optimum reaction conditions of reaction time 8 h,reaction temperature 71°c,and ratio of na2seo3 to sfpsib 1.0 g/g. a series of experiments showed that the characterization of se-sfpsib was significantly different from that of sfpsib. additionally,antioxidant activity assay indicated that the se-sfpsib could increase catalase (cat),superoxide dismutase (sod),and glutathione peroxidase (gsh-px) activity of mice bearing tumor s180 in blood,heart,and liver while decreasing malondialdehyde (mda) levels. it can be concluded that selenylation is a feasible approach to obtain seleno-polysaccharide which was utilized as highly biological medicine or functional food. © 2013 yu-bin ji et al.