The preparation of glucan-Fe3O4 magnetic nanoparticles and its in vivo distribution in mice

The glucan-fe3o4 magnetic nanoparticles were prepared by hydrothermal method. the mixture of fecl2 and glucan was stirred vigorously for half an hour under low temperature (15°c). koh of 1 mol/l was dropwise added,slowly,into the solution until the ph to 12. immediately,kno3 was added and the temperature was raised to 75°c for an hour. all the processes of fe3o4 crystal particles generation were under nitrogen. an atomic absorption spectrometry quantitative analysis method was built to determine the in vivo distribution of the glucan-fe3o4 magnetic nanoparticles in mice. the diameter of glucan-fe3o4 magnetic nanoparticles was about 25 nm and they were up taken by the liver primarily after intravenous administration via the tail. © 2014 fengdan jin.