A Validated HPLC-DAD Method for Simultaneous Determination of Etodolac and Pantoprazole in Rat Plasma

A simple,sensitive,and accurate hplc-dad method has been developed and validated for the simultaneous determination of pantoprazole and etodolac in rat plasma as a tool for therapeutic drug monitoring. optimal chromatographic separation of the analytes was achieved on a waters symmetry c18 column using a mobile phase that consisted of phosphate buffer ph4.0 as eluent a and acetonitrile as eluent b in a ratio of a: b,55: 45 v/v for 6 min,pumped isocratically at a flow rate of 0.8 ml min-1. the eluted analytes were monitored using photodiode array detector set to quantify samples at 254 nm. the method was linear with r 2 = 0.9999 for ptz and r 2 = 0.9995 for etd at a concentration range of 0.1-15 and 5-50 gml-1 for ptz and etd,respectively. the limits of detection were found to be 0.033 and 0.918 gml-1 for ptz and etd,respectively. the method was statistically validated for linearity,accuracy,precision,and selectivity following the international conference for harmonization (ich) guidelines. the reproducibility of the method was reliable with the intra- and interday precision (% rsd) <7.76% for ptz and <7.58 % for etd. © 2014 ali s. abdelhameed and samar a. afifi.