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Comparison of two methods for the extraction of fractionated rice bran protein
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نویسنده
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wang c. ,li d. ,xu f. ,hao t. ,zhang m.
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منبع
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journal of chemistry - 2014 - دوره : 2014 - شماره : 0
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چکیده
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Two different methods for extracting fractionated rice bran protein (frbp) from defatted rice bran were investigated according to the solubility of protein in different extraction solvents. the yields of the obtained proteins and their purity were first compared. sodium dodecyl sulfate polyacrylamide gel electrophoresis,differential scanning calorimetry,protein surface hydrophobicity,and protein secondary molecular structure analyses were supsequently applied to identify and compare the compositional,structural,and functional characteristics of the obtained proteins. the highest yield (13.8%,w/w) and purity (45-47%) of frbp products were obtained using 0.4 m nacl,80% ethanol,and 0.01 m naoh as extraction solvents to fractionate albumin,globulin,prolamin,and glutelin. several good properties were exhibited,including good functionality,specific denaturation temperature,and enthalpy values,for frbp products prepared by the above method. © 2014 changyuan wang et al.
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آدرس
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college of food science,northeast agricultural university,harbin,heilongjiang,china,college of food science,heilongjiang bayi agricultural university,daqing, China, college of food science,heilongjiang bayi agricultural university,daqing, China, college of food science,heilongjiang bayi agricultural university,daqing, China, college of food science,heilongjiang bayi agricultural university,daqing, China, college of food science,northeast agricultural university,harbin,heilongjiang,china,college of food science,beijing technology and business university,33 fucheng road, China
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Authors
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