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Construction of differential-methylation subtractive library
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نویسنده
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hu w. ,liang x. ,dong t. ,hu y. ,liu j. ,mao l. ,liu x. ,bi y.
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منبع
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journal of chemistry - 2014 - دوره : 2014 - شماره : 0
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چکیده
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Stress-induced ros changes dna methylation patterns. a protocol combining methylation-sensitive restriction endonuclease (ms-re) digestion with suppression subtractive hybridization (ssh) to construct the differential-methylation subtractive library was developed for finding genes regulated by methylation mechanism under cold stress. the total efficiency of target fragment detection was 74.64%. dna methylation analysis demonstrated the methylation status of target fragments changed after low temperature or dna methyltransferase inhibitor treatment. transcription level analysis indicated that demethylation of dna promotes gene expression level. the results proved that our protocol was reliable and efficient to obtain gene fragments in differential-methylation status. © 2014 wei hu et al.
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آدرس
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school of life sciences,lanzhou university, China, school of life sciences,lanzhou university, China, school of life sciences,lanzhou university, China, key laboratory of mollisols agroecology,northeast institute of geography and agroecology,chinese academy of sciences,nangang district, China, school of life sciences,lanzhou university, China, school of life sciences,lanzhou university, China, school of life sciences,lanzhou university, China, school of life sciences,lanzhou university, China
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Authors
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