Flavonoids Extraction from Taraxacum officinale (Dandelion): Optimisation Using Response Surface Methodology and Antioxidant Activity

The box-behnken design combined with response surface method was employed to optimize ultrasonic-assisted extraction of flavonoids from taraxacum officinale. the optimized results showed that the highest extraction yield with ultrasonic-assisted extraction could reach 2.62% using 39.6% (v/v) ethanol and 59.5: 1 (ml/g) liquid-solid ratio for 43.8 min. the crude extract was then purified by hpd-100 macroporous adsorption resin,and the flavonoids content in the purified extract increased to 54.7%. the antioxidant activity of the purified flavonoids was evaluated in vitro by scavenging capacity of abts or dpph,β-carotene bleaching,and ftc test. the knowledge obtained from this study should be useful to further develop and apply this plant resource. © 2014 zongxi sun et al.