A Benzochalcone Derivative,(E)-1-(2-hydroxy-6-methoxyphenyl)-3-(naphthalen-2-yl)prop-2-en-1-one (DK-512),Inhibits Tumor Invasion through Inhibition of the TNF -Induced NF- B/MMP-9 Axis in MDA-MB-231 Breast Cancer Cells

Tumor invasion is a critical step in tumor metastasis. in this study,we synthesized a novel benzochalcone derivative,(e)-1-(2-hydroxy-6-methoxyphenyl)-3-(naphthalen-2-yl) prop-2-en-1-one (dk-512),and characterized its effects on tumor invasion and its mechanism of action. we found that dk-512 strongly inhibited invasion of metastatic mda-mb-231 breast cancer cells as revealed by a three-dimensional spheroid culture system. tumor invasion and metastasis require disruption of the extracellular matrix. matrix metalloproteinase-9 (mmp-9) is an endopeptidase that degrades extracellular matrix components. dk-512 significantly reduced tumor necrosis factor- (tnf;-) induced mmp-9 mrna expression through the inhibition of rela nuclear factor- (nf-) b transcription factor. as our study was assessed in vitro,further works about in vivo efficacy of dk-512 are needed to gain further insights into whether dk-512 could be utilized as a scaffold for the development of antimetastatic agents for breast cancer. � 2016 soon young shin et al.