Determination of Cefoperazone Sodium in Presence of Related Impurities by Improved Classical Least Squares Chemometric Methods: A Comparative Study

A comparative study is established among 4 chemometric models depending on classical least squares (cls) approach,namely,spectral residual augmented cls (sracls),net analyte processing cls (nap-cls),orthogonal signal correction cls (osc-cls),and direct orthogonal signal correction cls (dosc-cls). the comparison is expressed through analysis of a case study dataset of uv spectral data of cefoperazone sodium (cef) and its two related impurities: in pure powder form and in pharmaceutical dosage form. four-level three-factor experimental design was established for optimum analysis. the adopted experimental design gave rise to a training set consisting of 16 mixtures (containing different ratios of interfering species). to test the prediction power of the suggested models,an independent test set consisting of 9 mixtures was used. the presented results show the ability of the proposed models to quantify cef in presence of two related impurities with high accuracy and selectivity (103.76 � 1.03,102.07 � 0.91,101.61 � 0.72,and 101.60 � 0.72 for sracls,nap-cls,osc-cls,and dosc-cls,resp.). dosage form analysis results were compared statistically to a published hplc methodology showing insignificant difference in terms of precision and accuracy,indicating the suggested models reliability and their suitability for quality control analysis of drug product. compared to other models,osc-cls and dosc-cls models gave more accurate results with lower prediction error for test set samples. � 2016 ibrahim a. naguib and hany w. darwish.