Physicochemical Characterisation of Polysaccharides from the Seeds and Leaves of Miracle Fruit (Synsepalum dulcificum) and Their Antioxidant and α-Glucosidase Inhibitory Activities in Vitro

Miracle fruit (synsepalum dulcificum) has been well known and studied for its unique taste-modifying ability. in this study,the monosaccharide composition,molecular weight (mw),and in vitro bioactivities (antioxidant,α-glucosidase inhibition) of polysaccharides from the seeds (mfp-s) and leaves (mfp-l) of miracle fruit were investigated. the results showed that mfp-s was a homogeneous polysaccharide (mw 2804 da) with glucose. mfp-l displayed three fractions (92093,1496,and 237 da) consisting of rhamnose,arabinose,galactose,glucose,and xylose. moreover,the antioxidant and α-glucosidase inhibition of mfp-l were significantly greater than those of mfp-s. the α-glucosidase inhibition of mfp-l was remarkably better than the positive control,acarbose (an antidiabetes drug). more specifically,the 50% inhibitory concentration (ic50) values of α-glucosidase activities for mfp-s,mfp-l,and acarbose were 33,0.01,and 1 mg ml-1,separately. therefore,mfp-l can be developed as a functional factor with both antioxidant and antidiabetes activities in food applications. © 2017 huajun jian et al.