EXPRESSION OF PEROXISOME-PROLIFERATOR ACTIVATED RECEPTORS-γ IN DIABETICS,OBESE AND NORMAL SUBJECTS

Background: current researcn in type 2 diabetes mellitus focuses on the role of peroxisome- proliferator activated receptors (ppars) in the pathogenesis of the insulin resistance syndrome (irs),which are pre-diabetic lesion and the hallmark of fully developed type 2 diabetes mellitus. this study aims at identifying the abnormal status of the ppar-γ in adipose tissues of type 2 diabetes mellitus patients,when compared with matched normal controls.methods: this cross-sectional study was conducted in ayub medical college,abbottabad,from 2012 to 2014. sample included three equal groups of patients. group-1 with diagnosed type 2 diabetes mellitus,aged 40-65 years,acting as the test group,group-2 included non-diabetic obese,and group-3 with normal subjects. transcription factor assay for peroxisome proliferator activated receptor gamma (gamma ppar) was done on elisa technique from nuclear extract procured from adipose tissue of the subjects.results: mean age of enrolled participants was 48.93 sd ± 6.52 years. patients ranged between ages of 40 years to 67 years. the mean values of ppar in normal,obese and diabetic group were 1.72 sd ± 0.28,1.282 se ± 0.18 and 1.283 se ± 0.18 respectively. the difference in mean values of ppar was significant p < 0.05:conclusion: the levels of ppar-γ in patients with type 2 diabetes mellitus and obese cases are significantly lower than normal controls.