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Simultaneous and direct determination of vancomycin and cephalexin in human plasma by using HPLC-DAD coupled with second-order calibration algorithms
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نویسنده
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hu l.-q. ,yin c.-l. ,du y.-h. ,zeng z.-p.
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منبع
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journal of analytical methods in chemistry - 2012 - دوره : 2012 - شماره : 0
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چکیده
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A simple,rapid,and sensitive method for the simultaneous determination of vancomycin and cephalexin in human plasma was developed by using hplc-dad with second-order calibration algorithms. instead of a completely chromatographic separation,mathematical separation was performed by using two trilinear decomposition algorithms,that is,parafac-alternative least squares (parafac-alss) and self-weight-alternative-trilinear-decomposition- (swatld-) coupled high-performance liquid chromatography with dad detection. the average recoveries attained from parafac-als and swatld with the factor number of 4 (n = 4) were 101 5 and 102 4 for vancomycin,and 96 3 and 97 3 for cephalexininde in real human samples,respectively. the statistical comparison between parafac-als and swatld is demonstrated to be similar. the results indicated that the combination of hplc-dad detection with second-order calibration algorithms is a powerful tool to quantify the analytes of interest from overlapped chromatographic profiles for complex analysis of drugs in plasma. © 2012 le-qian hu et al.
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آدرس
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school of chemistry and chemical engineering,henan university of technology, China, school of chemistry and chemical engineering,henan university of technology, China, school of chemistry and chemical engineering,henan university of technology, China, school of chemistry and chemical engineering,henan university of technology, China
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Authors
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