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Different stability-indicating chromatographic techniques for the determination of netobimin
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نویسنده
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ramadan n.k. ,mohamed a.o. ,shawky s.e. ,salem m.y.
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منبع
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journal of analytical methods in chemistry - 2012 - دوره : 2012 - شماره : 0
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چکیده
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Two simple,accurate,and sensitive methods were developed for the determination of netobimin in the presence of its degradation product. method (a) was an hplc method,performed on c18 column using acetonitrile/methanol/0. 01m potassium dihydrogen phosphate (56:14:30 by volume) as a mobile phase with a flow rate of 0.5ml/min. detection was performed at 254nm. method (b) was a tlc method,using silica gel 60 f254 plates; the optimized mobile phase was toluene/methanol/chloroform/ammonium hydroxide (5:4:6:0.1 by volume). the spots were scanned densitometrically at 346nm. linearity ranges were 1-10g/ml for method (a) and 0.5-5g/band for method (b),and the mean percentage recoveries were 99.3±0.7 % and 99.7±0.7 % for methods (a) and (b),respectively. the proposed methods were found to be specific for netobimin in the presence of up to 90% of its degradation product. statistical comparison between the results obtained by these methods and the manufacturer method was done,and no significance difference was obtained. copyright 2012 nesrin k. ramadan et al.
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آدرس
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analytical chemistry department,faculty of pharmacy,cairo university,kasr el-aini street, Egypt, analytical chemistry department,national organization for drug control and research (nodcar),6 abu hazem street,pyramids avenue,35521,egypt,department of pharmaceutical chemistry,faculty of pharmacy,umm al-qura university, Saudi Arabia, analytical chemistry department,national organization for drug control and research (nodcar),6 abu hazem street,pyramids avenue, Egypt, analytical chemistry department,faculty of pharmacy,cairo university,kasr el-aini street, Egypt
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Authors
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