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Quenching-chemiluminescence determination of trace amounts of l-tyrosine contained in dietary supplement by chemiluminescence reaction of an iron-phthalocyanine complex
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نویسنده
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ohtomo t. ,igarashi s. ,takagai y. ,ohno o.
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منبع
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journal of analytical methods in chemistry - 2012 - دوره : 2012 - شماره : 0
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چکیده
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The chemiluminescence (cl) signal immediately appeared when a hydrogen peroxide solution was injected into an iron-phthalocyanine tetrasulfonic acid (fe-pts) aqueous solution. moreover,the cl intensity of fe-pts decreased by adding l-tyrosine. based on these results,the determination of trace amounts of l-tyrosine was developed using the quenching-chemiluminescence. the calibration curve of l-tyrosine was obtained in the concentration range of 2.0 10 -7 m to 2.0 10 -5 m. moreover,the relative standard deviation (rsd) was 1.63 % (n=5) for 2.0 10 -6 m l-tyrosine,and its detection limits (3δ) were 1.81 10 -7 m. the spike and recovery experiments for l-tyrosine were performed using a soft drink. furthermore,the determination of l-tyrosine was applied to supplements containing various kinds of amino acids. each satisfactory relative recovery was obtained at 98 to 102%. copyright 2012 takao ohtomo et al.
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آدرس
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department of biomolecular functional engineering,faculty of engineering,ibaraki university,4-12-1 nakanarusawa,hitachi, Japan, department of biomolecular functional engineering,faculty of engineering,ibaraki university,4-12-1 nakanarusawa,hitachi, Japan, faculty of symbiotic systems science,cluster of science and technology,fukushima university,kanayagawa 1, Japan, department of biomolecular functional engineering,faculty of engineering,ibaraki university,4-12-1 nakanarusawa,hitachi, Japan
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Authors
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