Analysis of alphalactalbumin and betalactoglobulin from the rehydration of bovine colostrum powder using cloud point extraction and mass spectrometry

Alphalactalbumin (-la) and betalactoglobulin (-lg) in the rehydration of bovine colostrum powder were successfully separated by cloud point extraction using a nonionic surfactant triton x-114. the effects of different factors,including the surfactant concentration,sample volume,electrolyte,and ph were discussed. the optimized conditions for cloud point extraction of alphalactalbumin (-la) and betalactoglobulin (-lg) can be concluded that the best surfactant is 1 (w/v) triton x-114,200 l of sample volume,150 mmol/l nacl,and 6 (w/v) sucrose. after cloud point extraction,the capillary electrophoresis is used to check the efficiency of the extraction procedure. the results had been effectively confirmed by the characterization with matrix-assisted laser desorption ionization time of flight mass spectrometry (maldi-tof ms). copyright 2012 fan zhang et al.