Alsterpaullone,a cyclin-dependent kinase inhibitor,mediated toxicity in HeLa cells through apoptosis-inducing effect

Alsterpaullone,a small molecule cyclin-dependent kinase (cdk) inhibitor,regulates the cell cycle progression. beyond death-inducing properties,we identified the effect of alsterpaullone on cycle procedure and apoptosis of hela cell. it was found that alsterpaullone inhibited hela cells in a time-dependent (0-72 h) and dose-dependent (0-30 m) manner. in the presence of alsterpaullone,hela cells were arrested in gm prior to undergoing apoptosis via a mechanism that is involved in the regulation of various antiapoptotic genes,dna-repair,transcription,and cell cycle progression. compared to controls,alsterpaullone effectively prevented hela cells from entering s-phase. these potential therapeutic efficacies could be correlated with the activation of caspase-3. © 2013 chunying cui et al.