Determination of L-argininamide based on water-soluble fluorescent conjugated polymer-aptamer

Water-soluble fluorescent conjugated polymer is a promising material which could be used as an optical platform in highly sensitive molecular sensors. in this paper,a simple label-free dna sensor,which consisted of a poly(3-alkoxy-4-methylthiophene) and an aptamer,was used to detect l-argininamide (l-arm). due to the specific binding reaction between l-arm and its aptamer,the proposed method can easily determinate the l-arm through the recovery of fluorescence without any modification. other ions or similar molecules had little effect on the detection. moreover,there was a linear relationship between fluorescence intensity and the concentration of l-arm. the detection limit of l-arm was as low as 4.7 nm. © 2013 hongliang guan and zhike he.