Simultaneous determination of volatile constituents from acorus tatarinowii schott in rat plasma by gas chromatography-mass spectrometry with selective ion monitoring and application in pharmacokinetic study

A sensitive and specific gas chromatographic-mass spectrometry with selected ion monitoring (gc-ms/sim) method has been developed for simultaneous identification and quantification of α-asarone,β -asarone,and methyl eugenol of acorus tatarinowii schott in rat plasma. chromatographic separation was performed on a restek rxi-5ms capillary column (30 m × 0.32 mm × 0.25 m),using 1-naphthol as internal standard (is). ms detection of these compounds and is was performed at m/z 178,208,208,and 144. intra- and interday precisions of all compounds of interest were less than 10%. the recoveries are situated in the range of 92.4-105.2%. pharmacokinetics of methyl eugenol confirmed to be one-compartment open model,α-asarone and β-asarone was two-compartment open model,respectively. the method will probably be an alternative to simultaneous determination and pharmacokinetic study of volatile ingredients in acorus tatarinowii schott. © 2013 xue meng et al.