Simple and sensitive analysis of blonanserin and blonanserin C in human plasma by liquid chromatography tandem mass spectrometry and its application

A highly sensitive,simple,and rapid liquid chromatography tandem mass spectrometry method to simultaneously determine blonanserin and blonanserin c in human plasma with ad-5332 as internal standard (is) was established. a simple direct protein precipitation method was used for the sample pretreatment,and chromatographic separation was performed on a waters xbridge c8 (4.6×150 mm,3.5 m) column. the mobile phase consists of a mixture of 10 mm ammonium formate and 0.1% formic acid in water (a) and 0.1% formic acid in methanol (b). to quantify blonanserin,blonanserin c,and is,multiple reaction monitoring (mrm) was performed in positive esi mode. the calibration curve was linear in the concentration range of 0.012-5.78 ng·ml-1 for blonanserin and 0.023-11.57 ng·ml-1 for blonanserin c (r2>0.9990). the intra- and interday precision of three quality control (qc) levels in plasma were less than 7.5%. finally,the current simple,sensitive,and accurate lc-ms/ms method was successfully applied to investigate the pharmacokinetics of blonanserin and blonanserin c in healthy chinese volunteers. © 2014 yunliang zheng et al.