Isolation and bioactivity analysis of ethyl acetate extract from acer tegmentosum using in vitro assay and on-line screening HPLC-ABTS+ system

The acer tegmentosum (3 kg) was extracted using hot water,and the freeze-dried extract powder was partitioned successively using dichloromethane (dcm),ethyl acetate (ea),butyl alcohol (n-buoh),and water. from the ea extract fraction (1.24 g),five phenolic compounds were isolated by the silica gel,octadecyl silica gel,and sephadex lh-20 column chromatography. based on spectroscopic methods such as 1h-nmr,13c-nmr,and lc/ms the chemical structures of the compounds were confirmed as feniculin (1),avicularin (2),(+)-catechin (3),(-)-epicatechin (4),and 6′-o-galloyl salidroside (5). moreover,a rapid on-line screening hplc-abts+ system for individual bioactivity of the ea-soluble fraction (five phenolic compounds) was developed. the results indicated that compounds 1 and 2 were first isolated from the a. tegmentosum. the anti-inflammatory activities and on-line screening hplc-abts+ assay method of these compounds in lps-stimulated murine macrophages were rapid and efficient for the investigation of bioactivity of a. tegmentosum. © 2014 kwang jin lee et al.