A comparative study of newly developed HPLC-DAD and UHPLC-UV assays for the determination of posaconazole in bulk powder and suspension dosage form

Objective. to develop and compare hplc-dad and uhplc-uv assays for the quantitation of posaconazole in bulk powder and suspension dosage form. methods. posaconazole linearity range was 5-50 g/ml for both assays. for hplc-dad assay,samples were injected through zorbax sb-c18 (4.6 × 250 mm,5 m) column. the gradient elution composed of the mobile phase acetonitrile: 15 mm potassium dihydrogen orthophosphate (30: 70 to 80: 20,linear over 7 minutes) pumped at 1.5 ml/min. for uhplc-uv assay,samples were injected through kinetex-c18 (2.1 × 50 mm,1.3 m) column. the mobile phase composed of acetonitrile: 15 mm potassium dihydrogen orthophosphate (45: 55) pumped isocratically at 0.4 ml/min. detection wavelength was 262 nm in both methods. results. the run time was 11 and 3 minutes for hplc-dad and uhplc-uv assays,respectively. both assays were linear (r 2 > 0.999) with cv% and % error of the mean <3%. limits of detection and quantitation were 0.82 and 2.73 g/ml for hplc-dad and 1.04 and 3.16 g/ml for uhplc-uv,respectively. the methods quantitated psz in suspension dosage form with no observable interferences. conclusions. both assays were proven sensitive and selective according to ich guidelines. uhplc-uv assay exhibited some economic and chromatographic separation superiority. © 2014 dalia a. hamdy and tarek s. belal.