Expression of recombinant human alpha-lactalbumin in the milk of transgenic goats using a hybrid pomoter/enhancer

To improve nutrient content of goat milk,we describe the construction of a vector (pblac) containing a hybrid goat β-lactoglobulin (blg) promoter/cytomegalovirus (cmv) enhancer. we also describe the generation of transgenic goats expressing rhla by somatic cell nuclear transfer (scnt). of 334 one-cell stage embryos derived from three transgenic cell lines and 99 embryos derived from non-transgenic (nt) cells surgically transferred to the oviducts of 37 recipients,two recipients delivered two kids (2%) from the non-transfected line and five recipients delivered six kids (1.8%) from transgenic cell lines,three of which died within 2 days. compared to the nt donor cells,transfection of donor cells does not negatively affect the development of nuclear transfer embryos into viable transgenic offspring. however,the clone efficiency in cell line number 1 was lower than that in numbers 2 and 3,and in the nt lines (0.9% versus 1.9% 2.4% and 2%; p < 0.05). two transgenic cloned goats expressed rhla in the milk at 0.1-0.9 mg/ml. the mammary gland-specific expression vector pblac with hybrid blg/cmv can drive the hla gene to express in vitro and in vivo. these data establish the basis for use of a hybrid promoter/enhancer strategy to produce rhla transgenic goats. © 2014 yu-guo yuan et al.