Development of a method to detect three monohydroxylated polycyclic aromatic hydrocarbons in human urine by liquid chromatographic tandem mass spectrometry

A liquid chromatographic tandem mass spectrometry method (lc-ms/ms) for the simultaneous determination of 1-hydroxypyrene (1-ohp),3-hydroxybenzo[a]pyrene (3-ohbap),and 3-hydroxybenz[a]anthracene (3-ohbaa) in human urine has been developed. with the exception of 3-ohbap at a low spiking level,the average recoveries were greater than 80%. the method has good accuracy (72.1-107.7%) and reproducibility (1.8-11.4%) and was successfully used to study the uptake of pyrene,benzo[a]pyrene,and benzo[a]anthracene from cigarette smoke. the results indicated that urinary 1-ohp concentration in the smoking group (66.58 ± 70.91 ng/g creatinine) was higher than that observed in the nonsmoking group (58.16 ± 49.48 ng/g creatinine). urinary 3-ohbaa concentrations in nonsmokers and smokers with 8 mg and 10 mg tar cigarettes were 10.98 ± 4.39 ng/g creatinine,11.01 ± 13.30 ng/g creatinine,and 9.17 ± 12.89 ng/g creatinine,respectively. urinary 3-ohbap concentrations in nonsmokers and smokers with 8 mg and 13 mg tar cigarettes were 1.30 ± 0.20 ng/g creatinine,2.83 ± 1.78 ng/g creatinine,and 6.00 ± 4.44 ng/g creatinine,respectively. urinary 1-ohp levels exhibited a significant correlation with bap yield in cigarette smoke under the canadian intense smoking condition (y = 3.5563 x + 30.171,r 2 = 0.9916,n = 227). © 2015 xiaotao zhang et al.