Determination of matrine in rat plasma after oral administration of novel korean herbal medicine KIOM-MA128 and application of PK

Kiom-ma128 is a novel korean herbal medicine with antiatopic,anti-inflammatory,and antiasthmatic effects. matrine is thought to be a potential chemical marker of kiom-ma128,but pharmacokinetic studies on kiom-ma128 had not been performed. this study describes a simple and rapid method using high-performance liquid chromatography-tandem mass spectrometry (hplc-ms/ms) to determine the concentration of matrine in rats plasma after administration of kiom-ma128. the isocratic mobile phase consisted of methanol and distilled water,and the flow rate was 0.15 ml/min. the accuracy and precision of the assay,as well as stability tests,were performed in accordance with fda regulations for the validation of bioanalytical methods. the half-life and t max of matrine after administration of kiom-ma128 were 4.29 ± 2.20 h and 1.8 ± 1.23 h,respectively. c max and auc inf of matrine after administration of kiom-ma128 at 4 g/kg and 8 g/kg were 595.10 ± 182.91 ng/ml,5336.77 ± 1503.84 ng/ml·h and 850.46 ± 120 ng/ml,9583.10 ± 888.92 ng/ml·h,respectively. the validated method was successfully applied to a pharmacokinetic study in rats after oral administration of kiom-ma128. © 2015 hyun-moon back et al.