Developing a Novel Indolocarbazole as Histone Deacetylases Inhibitor against Leukemia Cell Lines

A novel indolocarbazole (named as zw2-1) possessing hdac inhibition activity was synthesized and evaluated against human leukemia cell lines hl-60 and nb4. zw2-1 performed anti-population growth effect which was in a concentration-dependent manner (2-12 μm) by inducing both apoptosis and autophagy in cells. the compound also caused differentiation of hl-60 and nb4 cells as shown by increasing expression of cd11b,cd14,and cd38 at moderate concentration (4 μm). at relatively high concentration (8 μm),zw2-1 significantly decreased intracellular histone deacetylase 1 level which was also observed. all the results indicated that zw2-1 could be a novel antileukemia lead capable of simultaneously inducing apoptosis,autophagy,and differentiation. © 2015 wenjing wang et al.