Synthesis and Characterization of Molecular Imprinting Polymer Microspheres of Piperine: Extraction of Piperine from Spiked Urine

Molecularly imprinted polymer (mip) microspheres for piperine were synthesized by precipitation polymerization with a noncovalent approach. in this research piperine was used as a template,acrylic acid as a functional monomer,ethylene glycol dimethacrylate as a cross-linker,and 2,2′-azobisisobutyronitrile (aibn) as an initiator and acetonitrile as a solvent. the imprinted and nonimprinted polymer particles were characterized by using fourier transform infrared spectroscopy (ft-ir) and scanning electron microscopy (sem). the synthesized polymer particles were further evaluated for their rebinding efficiency by batch binding assay. the highly selected imprinted polymer for piperine was mip 3 with a composition (molar ratio) of 0.5: 3: 8,template: monomer: cross-linker,respectively. the mip 3 exhibits highest binding capacity (84.94%) as compared to other imprinted and nonimprinted polymers. the extraction efficiency of highly selected imprinted polymer of piperine from spiked urine was above 80%. © 2016 rachel marcella roland and showkat ahmad bhawani.