Comparative liquid chromatographic study for concurrent determination of canagliflozin and metformin in combined tablets

New hplc-uv method (method a),for simultaneous determination of metformin (met) and canagliflozin (cana),was developed and compared to another novel uplc-uv method (method b) in their tablet combination. concerning method a,isocratic separation was done by c18 column (100mm × 2.1 mm,3 μm) using methanol and 0.03m phosphate buffer (75: 25,v/v) at ph 3.2 as a mobile phase. meanwhile,chromatographic separation in method b was achieved via hypersil® gold (50mm × 2.1 mm,1.9 μm). mobile phase was methanol and 0.03m phosphate buffer at ratio of 80: 20 v/v. in both,detection was done at wavelength of 240 nm. method a showed satisfactory linearity results over 1-50 μg · ml-1 and 0.5-100 μg · ml-1,while method b linearitywas at 0.1-50 μg · ml-1 and 0.25-100 μg · ml-1 for canaandmet,respectively. in terms of accuracy and precision,method a accuracy was 99.81 ± 0.73 and 99.37 ± 0.54,while method b gave accuracy of 99.47 ± 1.03 and 99.73 ± 0.89 for cana andmet,respectively. for precision,the % rsd was found to be less than 2% for three concentrations analyzed three times.the two methods are convenient for quality laboratories,yet the uplc method offered the advantage of shorter run times and higher sensitivity. © copyright 2017 wafaa a. zaghary et al.