UPLC-tandem mass spectrometry method for simultaneous determination of fluoxetine,risperidone,and its active metabolite 9-hydroxyrisperidone in plasma: Application to pharmacokinetics study in rats

Risperidone (ris) is used as an antipsychotic drug alone or with other drugs,like fluoxetine (flx). a simple method was developed and validated for the determination of both ris and its metabolite 9-hydroxyrisperidone (9-oh-ris),flx,and olanzapine (ola) as an internal standard in rat's plasma using uplc-ms/ms. flx,ris,9-oh-ris,and ola were purified using acetonitrile as a protein precipitating agent. separation was performed on an acquity™ uplc beh™ c18 column (50 mm × 2.1 mm i.d.,1.7 μm; waters corp.,usa). the ranges of the calibration curves were 1.0-1000.0 ng/ml for flx and 0.2-1000.0 ng/ml for ris and 9-oh-ris. linearity,recovery,precision,and stability were within the acceptable range. this method is rapid,fast,and precise for the determination of ris and flx in plasma and is applicable in pharmacokinetic studies. © 2017 essam ezzeldin et al.