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Semiautomated Alignment of High-Throughput Metabolite Profiles with Chemometric Tools
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نویسنده
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wu z.-y. ,zeng z.-d. ,xiao z.-d. ,mok d.k.-w. ,liang y.-z. ,chau f.-t. ,chan h.-y.
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منبع
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journal of analytical methods in chemistry - 2017 - دوره : 2017 - شماره : 0
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چکیده
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The rapid increase in the use of metabolite profiling/fingerprinting techniques to resolve complicated issues in metabolomics has stimulated demand for data processing techniques,such as alignment,to extract detailed information. in this study,a new and automated method was developed to correct the retention time shift of high-dimensional and high-throughput data sets. information from the target chromatographic profiles was used to determine the standard profile as a reference for alignment. a novel,piecewise data partition strategy was applied for the determination of the target components in the standard profile as markers for alignment. an automated target search (ats) method was proposed to find the exact retention times of the selected targets in other profiles for alignment. the linear interpolation technique (lit) was employed to align the profiles prior to pattern recognition,comprehensive comparison analysis,and other data processing steps. in total,94 metabolite profiles of ginseng were studied,including the most volatile secondary metabolites. the method used in this article could be an essential step in the extraction of information from high-throughput data acquired in the study of systems biology,metabolomics,and biomarker discovery. © 2017 ze-ying wu et al.
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آدرس
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school of mathematics,physics and chemical engineering,changzhou institute of technology,changzhou,china,state key testing laboratory of food contact materials,changzhou entry-exit inspection and quarantine bureau,changzhou, China, chemometrics and herbal medicine laboratory,department of applied biology and chemical technology,hong kong polytechnic university,hung hom,kowloon,hong kong,dalian chemdatasolution technology co. ltd.,high-tech zone,dalian,liaoning, China, school of chemical and biological engineering,changsha university of science and technology,changsha, China, chemometrics and herbal medicine laboratory,department of applied biology and chemical technology,hong kong polytechnic university,hung hom,kowloon,hong kong,state key laboratory of chinese medicine and molecular pharmacology,shenzhen, China, research center of modernization of chinese medicines,college of chemistry and chemical engineering,central south university,changsha, China, chemometrics and herbal medicine laboratory,department of applied biology and chemical technology,hong kong polytechnic university,hung hom,kowloon,hong kong,state key laboratory of chinese medicine and molecular pharmacology,shenzhen, China, school of chemical and biological engineering,changsha university of science and technology,changsha, China
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Authors
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