The Evidence of Cooperative Binding of a Ligand to G4 DNA

Intrinsic constants of the ligand binding with g4 dna (guanine-rich dna sequence) using quantitative standards can be convenient providing the assessment for elucidating the possibility of such structures participation in biochemical processes. in the present communication,the hard + soft modelling approach to calculate intrinsic constants of a ligand binding with short dna molecule,particularly such as g4 dna,has been proposed. the suggested approach has focused upon the quantitative evaluating of a mutual influence between sites and between bound ligands. the cross-validation between a new hard + soft modelling and conventional stepwise complex formation algorithm has been conducted. a number of simulated examples will illustrate the methodology. the experimental mole-ratio titration of tmpyp4 by g4 dna [(cg3)2cgc(ag3)2g] has been reexamined. the [(cg3)2cgc(ag3)2g] that folds from a g-rich sequence found in the promoter region of c-kit oncogene can be considered as a molecule with two equivalent mutually influence binding sites. © 2017 a. g. kudrev.