Simultaneous Analysis of Ursolic Acid and Oleanolic Acid in Guava Leaves Using QuEChERS-Based Extraction Followed by High-Performance Liquid Chromatography

In this paper,a novel method of quechers-based extraction coupled with high-performance liquid chromatography has been developed for the simultaneous determination of ursolic acid (ua) and oleanolic acid (oa) in guava leaves. the quechers-based extraction parameters,including the amount of added salt,vortex-assisted extraction time,and absorbent amount,and the chromatographic conditions were investigated for the analysis of ua and oa in guava leaves. under the optimized conditions,the method showed good linearity over a range of 1-320 μg ml-1,with correlation coefficients above 0.999. the limits of detection of ua and oa were 0.18 and 0.36 μg ml-1,respectively. the intraday and interday precision were below 1.95 and 2.55%,respectively. the accuracies of the ua and oa determinations ranged from 97.4 to 111.4%. the contents of ua and oa in the guava leaf samples were 2.50 and 0.73 mg g-1,respectively. these results demonstrate that the developed method is applicable to the simultaneous determination of ua and oa in guava leaves. © 2017 chang xu et al.