In Situ Synthesis of a Magnetic Graphene Platform for the Extraction of Benzimidazoles from Food Samples and Analysis by High-Performance Liquid Chromatography

A novel method was proposed for the determination of five benzimidazoles (oxfendazole,mebendazole,flubendazole,albendazole,and fenbendazole) using magnetic graphene (g-fe3o4). g-fe3o4 was synthesized via in situ chemical coprecipitation. the properties of g-fe3o4 were characterized by various instrumental methods. g-fe3o4 exhibited a great adsorption ability and good stability towards analytes. various experimental parameters that might affect the extraction efficiency such as the amount of g-fe3o4,extraction solvent,extraction time,and desorption conditions were evaluated. under the optimized conditions,a method based on g-fe3o4 magnetic solid-phase extraction coupled with high-performance liquid chromatography was developed. a good linear response was observed in the concentration range of 0.100-100 μg/l for the five benzimidazoles,with correlation coefficients ranging from 0.9966 to 0.9998. the limits of detection (s/n=3) of the method were between 17.2 and 32.3 ng/l. trace benzimidazoles in chicken,chicken blood,and chicken liver samples were determined and the concentrations of oxfendazole,mebendazole,flubendazole,and fenbendazole in these samples were 13.0-20.2,1.62-4.64,1.94-6.42,and 0.292-1.04 ng/g,respectively. the recovery ranged from 83.0% to 115%,and the relative standard deviations were less than 7.9%. the proposed method was sensitive,reliable,and convenient for the analysis of trace benzimidazoles in food samples. © 2017 qianchun zhang et al.