molecular rapid diagnostic test for nosocomial non-fermenting gram-negative bacilli

Accurate diagnosis of nosocomial infections is crucial for appropriate antibiotic therapy, as well as avoiding unnecessary use of drugs. mainly 3 gram-negative bacteria including pseudomonas aeruginosa, acinetobacter baumannii, and stenotrophomonas maltophilia are involved in nosocomial infections. these bacteria are possibly misdiagnosed with together in traditional diagnostic methods. in this regard, a rapid molecular diagnostic kit was developed in shiraz burn research center. using this diagnostic kit, the sensitivity and specificity analyses showed following results, respectively: p. aeruginosa: 100%, 96.8%; a. baumannii: 100%, 100%, and s. maltophilia: 96.7%, and 93.8%. therefore, this multiplex polymerase chain reaction (pcr) method with 3 primer sequences could be a responsive technique in timely and appropriate detection of studied bacteria.