characterization of staphylococcus aureus isolated from wound infectious in diabetes clinic of hazrat fatemeh zahra (sa) hospital

Background: staphylococcus aureus is a gram positive pathogen which causes a wide range of infections. the present study aimed to investigate genotypic and phenotypic screening about biofilm formation in s. aureus isolated from wound infections in a diabetes clinic in hazrat fatemeh zahra (sa) hospital. methods: a total of 267 clinical samples were collected from various types of wound infections in the diabetes clinic of hazrat fatemeh zahra (sa) hospital, isfahan, iran. the methicillin-resistant s. aureus (mrsa) isolates were selected and biofilm formation and its related genes were analyzed by polymerase chain reaction (pcr). results: the results showed that 95 out of 132 samples were mrsa. the high resistance was seen to methicillin, erythromycin, ciprofloxacin, and penicillin. phenotypic results showed that 48.3% of the isolates were high biofilm producers, 29.1% were average biofilm producers, and 10.6% were low biofilm producers. conclusions: according to the results of this study, the expression levels of biofilm-associated genes significantly increased, and high prevalence of antibiotic resistance was one of the important reasons for the development of drug resistance in patients.