investigation of the microbial contamination of the hands of healthcareworkers in differentwards of sheikh hospital in mashhad

Background: many nosocomial infections, which cause death and cost society, may be transmitted through healthcare workers’ contacts. preventive health measures greatly reduce their prevalence. objectives: this study aimed to investigate the microbial contamination of healthcare workers’ hands in different wards of sheikh hospital. in addition, the alcohol-based hand sanitizers’ ability to reduce microbial load of nosocomial infections was evaluated. methods: the present study was performed in spring 2019. thirty-two nurses’ hand samples were obtained from different wards of the hospital, including emergency, icu, surgery, peritoneal dialysis, nephrology, and hematology-oncology. biochemical tests determined the isolates. participants’ hands were cleaned using a standard procedure using soap and 70% isopropyl alcohol-based hand sanitizer. results: different species, including coagulase-negative staphylococcus (cons), klebsiella pneumoniae, staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, bacillus, klebsiella oxytoca, acinetobacter baumannii, stenotrophomonas maltophilia, and burkholderia cepacia complex were isolated. following hand hygiene procedure, the most effective decontamination for a wide variety of organisms was observed. conclusion: separation of dangerous pathogenic bacteria such as s. aureus, k. pneumoniae, and e. coli from healthcare workers may be a great warning sign for these infections in the hospital. therefore, hand hygiene procedures may be considered an appropriate method to decrease nosocomial infections.