in silico b cell and t cell epitopes evaluation of lipl32 and ompl1 proteins for designing a recombinant multi-epitope vaccine against leptospirosis

Leptospirosis is a widespread zoonotic disease caused by leptospira interrogans. the conventional vaccines have some major problems. therefore, recombinant vaccines such as multiple-epitope vaccine are suggested. ompl1 and lipl32 are the most important proteins of leptospira interrogans bacteria that canbeused in epitope prediction process to design a multiple-epitope vaccine. hence, in this study, the most reliable and accurate online servers were applied to predict b cell and t cell epitopes, the secondary and tertiary structures, enzyme digestion, and antigenicity score of ompl1 and lipl32. the results showed that epitopes located at 103 - 122, 210 - 232, and 272 - 291 amino acid residues are the common epitopes between t cell (mhci) and b cell. 288 - 308 amino acid residues were introduced as common epitopes to stimulate both t cell (mhci and mhcii) and b cell of ompl1 protein. in the case of lipl32 protein, 80 - 96 amino acid residues are recommended for t cell epitopes and 63-81 amino acid residues for stimulation of both b and t cells. all the mentioned epitopes can be considered as linear epitopes in designing a recombinant vaccine based on chimeric epitopes. it appears that these epitopes can be applied to design recombinant multiple-epitope vaccines against leptospirosis.