the effect of artemisia sieberi (dermeneh dashti) plant extract on the expression of genes involved in the apoptosis signaling pathway in the ht_29 cell line

Background: colorectal cancer (crc) is one of the most common malignant tumors in humans. the influence of natural plant compounds is widely acknowledged today. artemisia sieberi has anti-bacterial, anti-fungal, and anti-inflammatory properties. objectives: the aim of the forthcoming study is to investigate the impact of artemisia sieberi plant extract on the expression of key genes within the apoptosis signaling pathway of the ht-29 cell line. methods: in this study, rna extraction and cdna synthesis were conducted subsequent to the culturing and treatment of ht-29 cancer cells with plains herb extract. a real-time pcr test utilizing specific primers for bax and bcl-2 was then performed using the rotor-gene q machine. additionally, an mtt assay was conducted to assess the toxicity of the artemisia sieberi extract. results: the test revealed that as the concentration of the studied extract increased, cell viability decreased in a dose-dependent manner. the calculated ic50 value for the plains herb extract was found to be 0.44 µg/ml. furthermore, the levels of bax and bcl-2 genes in cells treated with the plains herb extract were determined to be 1.48 and 0.45, respectively, indicating an increase in the expression of these two genes. conclusions: given the low toxicity of the artemisia sieberi extract observed in the mtt test and its potential to increase the expression of apoptosis-inducing genes in intestinal cells, it appears that artemisia sieberi extract could be employed as a therapeutic supplement for the prevention and treatment of gastrointestinal cancers.