epigenetic peculiarity of alphapapillomavirus genus based on the cpg dinucleotides’ island variability

Background: methylation plays a crucial role in genome regulation, serving as an essential epigenetic mechanism. cpg islands, which are regions of dna rich in cpg dinucleotides, are ubiquitous epigenetic regulatory features that can modulate the functional dynamics of genes, thereby influencing the pathophysiology of pathogenic organisms within a host.objectives: in this study, we aimed to investigate the distribution of cpg islands on papillomavirusgenomes and assess their potential impact on the neoplastic progression of different alphapapillomavirusgenera within host cells. methods: we conducted an analysis of dinucleotide frequencies in dna sequences from various alphapapillomavirusgenera. the sequences for our analysis were sourced from the specialized hpv (human papillomavirus) database (pave.niaid.nih.gov). following a comprehensive comparative examination of entire genomes from different hpv species, we specifically focused on 63 genomes belonging to the alphapapillomavirusgenus to identify internal cpg island profiles. these investigations were conducted using online bioinformatics software (dbcat), and statistical analysis was performed using graphpad prism v. 8.0.1. results: our preliminary findings revealed that 76.1% of the viruses in our study had fewer than 3 cpg islands but multiple cpg sites, while fewer than 25% of the viruses possessed at least 3 cpg islands. high-risk viruses were identified in 68.42% of genotypes with fewer than 3 cpg islands, whereas low-risk genotypes were observed in 15.7% of cases. notably, some oncogenic viruses lacked cpg islands entirely, and this pattern was also observed in viruses with a single cpg island, occurring in 50% of cases. based on the absence of cpg islands, genotypes such as hpv 67, hpv 97, and hpv 34 could potentially be classified as carcinogenic. additionally, the distribution of cpg islands across hpv genes did not appear to be random. genes like l2 and e2 contained a cpg island in 50% of cases, whereas oncogenes e6 and e7 consistently had a cpg island in 100% of cases in high-risk genotypes. conclusions: our findings suggest that the loss of 1 or more cpg islands could potentially transform an hpv genotype into a high-risk genotype. this process may be accelerated in the presence of host-related risk factors. further research is required to validate whether cpg island distribution can aid in identifying oncogenic viruses.