evaluation of nlr family card domain containing 3 and nlr family card domain containing 5 gene expression in interferon gamma–treated mesenchymal stem cells from wharton’s jelly of human umbilical cord

Background: human umbilical cord wharton’s jelly has provided a new source for mesenchymal stem cells (mscs). the highly proliferative capacity with low immunogenicity and multi-differentiation potential of its stem cells make them applicable for transplantation purposes. nucleotidebinding oligomerization domain (nod)-like receptors (nlrs) play various roles in antigen presentation of pathogens and damaged cells to suppress and/or modulate inflammation. objectives: in this study, the expression levels of nlr family card domain containing 3 (nlrc3) and nlrc5 genes were analyzed and compared in both untreated and interferon gamma (ifnγ)–treated wharton’s jelly-derived mscs (wj-mscs). methods: mscs were isolated from human umbilical cord wharton’s jelly using standard tissue culture. the expression of nlrc5 and nlrc3 genes was analyzed in ifnγ–treated wj-mscs (24 hours after treatment) and untreated wj-mscs (as a control) using quantitative realtime polymerase chain reaction (pcr). results: it was found that ifnγ treatment mimicking an inflammation scenario led to a statistically significant increase of nlrc3 and nlrc5 gene expression compared to untreated wjmscs (p ≤ 0.05). conclusions: it seems that higher expression of nlrc3 and nlrc5 genes in treated wjmscs may make them a proper candidate to be used as a source for cell therapy in inflammatory conditions.