effect of aerobic training and vitamin d consumption on nfatc1 gene expression in bone tissue of rats exposed to h2o2

Background: increased reactive oxygen species disrupt the balance between osteoblasts and osteoclasts. although the role of training (t) and vitamin d (vd) consumption in bone health has been shown, there is no accurate information on the role of these two interventions on the nuclear factor of activated t-cells, cytoplasmic 1 (nfatc1), as an osteoclast marker. objectives: therefore, this study aimed to examine the effect of t and vd on nfatc1 gene expression in bone tissue of rats exposed to h2o2. methods: fifty adult male wistar rats aged 8 - 10 weeks and weighing 180 to 220 g were randomly assigned to 10 groups including (1) control (c), (2) dimethyl sulfoxide + normal saline (sham; sh), (3) 1 mmol/kgh2o2 (1h), (4) 1h+vd, (5) 1h+t, (6) 1h+vd+t, (7) 2 mmol/kg h2o2 (2h), (8) 2h+vd, (9) 2h+t, and (10) 2h+vd+t. the research protocol lasted eight weeks to implement. the levels of nfatc1 gene expression were measured by qrt-pcr. results: based on the results, 1h and 2h significantly increased nfatc1 gene expression levels (p = 0.001). however, t (p = 0.001), vd (p = 0.001), and vd+t (p = 0.001) reduced nfatc1 gene expression in the bone tissue of rats exposed to 1 and 2 mmol h2o2. also, nfatc1 gene expression was significantly lower in the 1h+vd+t group than in the 2h + vd group (p = 0.03). conclusions: it seems that t and vd consumption both alone and synergistically have a reducing effect on nfatc1 as an osteoclast index in rats exposed to 1 and 2 mmol/kg h2o2.