microrna prediction in the fviii gene locus: a step towards hemophilia a control

Background: various mutations in factor viii (f8) gene locus are led to an x-linked bleeding disorder in patients with hemophilia a. one of the leading causes of inefficient treatment available for hemophilia a is the lack of specific and sensitive diagnostic procedure for the disease. the discovery of a functional role of micrornas (mirnas) in the pathogenesis of a wide range of human diseases makes them the potential, non-invasive, biomarker candidates for hemophilia a. therefore, advances in computational tools for mirna discovery leads to numerous recent publications on mirnas as putative biomarkers. objectives: the current study aimed at scanning the f8 gene region to predict novel mirnas as regulators of the f8 gene. methods: the potential of the fviii locus to express new mirnas was studied via reliable bioinformatics databases, such as sscprofiler, rnafold, mireval, mir-find, fommir, ucsc genome browser, and mirbase. results: data analysis from previously mentioned databases offered two stem-loop structures predicted to express novel mirnas. conclusions: the presented stem-loop structures can be used as powerful non-invasive biomarkers in early diagnosis of the disease and regulation of the factor viii gene after subsequent experimental verification.