the effects of preconditioning on survival of mesenchymal stem cells in vitro

Background: mesenchymal stem cells (mscs) survival decreased following in vivo injection and its application has been limited in stem cell therapy. preconditioning was established as a strategy to increase the cells’ efficiency, but more studies are necessary to determine its safety and mechanisms. objectives: the aim of this study was to evaluate the effect of preconditioning mesenchymal stem cells with low serum and h2o2 on in vitro cell survival. methods: mesenchymal stem cells were cultured and preconditioned with low serum and h2o2 for 6, 12, 24 and 48 hours in six groups. control, group i with 5 µm h2o2 , group ii with 10 µm h2o2 , group iii with 0.5% fetal bovine serum (fbs), group iv: 5 µm h2o2 + 0.5% fbs and v: 10 µm h2o2 + 0.5% fbs. cell proliferation was evaluated with the mtt assay. results: cell proliferation in groups iv and v increased significantly compared to the other groups after 6, 12 and 24 hours of treat- ment (p < 0.05). also, after 48 hours, cell treatment with 5 µm h2o2 + 0.5% fbs led to a significant increase in cell proliferation (p < 0.05). conclusions: our data suggest that preconditioning with 5 µm h2o2 + 0.5% fbs and 10 µm h2o2 + 5% fbs improved cell proliferation and viability. however, the mechanisms related to protective properties of preconditioning and using this strategy in stem cell therapy requires more research.