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   Simple and Sensitive High Performance Liquid Chromatographic Method ForThe Simultaneous Quantitation of the Phenylalanine in Human Plasma  
   
نویسنده Danafar Hossein ,Danafar Hossein ,Hamidi Mehrdad
منبع Pharmaceutical And Biomedical Research - 2015 - دوره : 1 - شماره : 3 - صفحه:11 -19
چکیده    Phenylalanine (phe) is the most reliable indicator for the diagnosis of phenylketonuria (pku).the purpose of this study is to establish a reliable and quick method for the assignment of phein peripheral capillary blood from newborns and children by high performance liquidchromatography with ultraviolet detection (hplc-uv). phenylketonuria (pku) is an inbornerror of metabolism characterized by the inability of the body to use phenylalanine (phe). arapid and sensitive high performance liquid chromatographic (hplc) method has beendeveloped for determination of phenylalanine in plasma. the method uses a proteinprecipitation step with sulfosalicilic acid for sample preparation by separation on a nova-packc18 column using sodium acetate buffer and acetonitrile (94: 6 v/v) adjusted to ph 6.5 withglacial acetic acid. the eluted peaks detected by a uv detector was set at wavelength of 215nm. the method was validated in the range of phenylalanine concentrations from 0.1 to20μg/ml. the limits of detection (lod) and quantitation (loq) of the method were 0.05 and0.1μg/ml, respectively. the average drug recovery from plasma was 88.60 percent throughoutthe linear concentration range., with the average within-run and between-run accuracy valuesof 103.3 and 115.350, respectively. the method is quick, easy, very steady and precise for thescreen, assignment, and evaluation of phe in human plasma by hplc, which is particularly auseful way for screening and diagnosis of pku and monitoring of a diet therapy.
کلیدواژه Phenylketonuria (Pku) ,Phenylalanine (Phe) Assay ,Reversed-Phase Hplc.
آدرس Zanjan University Of Medical Sciences, Zanjan Pharmaceutical Nanotechnology Research Center, ایران. Zanjan University Of Medical Sciences, School Of Pharmacy, Department Of Medicinal Chemistry, ایران, Zanjan University Of Medical Sciences, Zanjan Pharmaceutical Nanotechnology Research Center, ایران. Zanjan University Of Medical Sciences, School Of Pharmacy, Department Of Medicinal Chemistry, ایران, Zanjan University Of Medical Sciences, School Of Pharmacy, Department Of Pharmaceutics, ایران
 
     
   
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