synthesis and characterization of ciprofloxacin-loaded chitosan nanoparticles for eradication of pseudomonas aeroginosa biofilm

Background: pseudomonas aeruginosa biofilm is one of the problems in antibiotic treatment of infections. nanomedicines, such as chitosan (cs) can carry multiple drugs and improve the therapeutic effects of antibiotics.objectives: this study aimed at the synthesis and characterization of ciprofloxacin-loaded chitosan nanoparticles for eradication of p. aeroginosa biofilm.methods: cipro-cs microparticles were prepared by ionic gelation method and their size, zeta potential, and drug release pattern were determined. mbec and mbic of different groups of antibiotics (ciprofloxacin, ciprofloxacin-paβn, cs ciprofloxacin, and cs ciprofloxacin-paβn) were performed on biofilm samples of p. aeroginosa. results: ciprofloxacin loading efficiency was 35.51%, and encapsulation efficiency was 55.06%. released ciprofloxacin from cs nanoparticles was 80% after 24 hours. biofilm production was positive in 96.7% of the isolates while 30.1% of the samples had strong biofilm. the best result for mbic was cs ciprofloxacin, cs ciprofloxacin-paβn, ciprofloxacin- paβn, and ciprofloxacin, respectively. for mbec the result was slightly different and from the best to better cs ciprofloxacin-paβn, cs ciprofloxacin, ciprofloxacin-paβn, and ciprofloxacin.conclusion: today, with increasing antibiotic resistance, there are many challenges in treating infections. due to the role of biofilm in antibiotic resistance, researchers are looking for new antibiotics to treat infections.