a comparative study of apigenin content and antioxidant potential of cosmos bipinnatus transgenic root culture

Flavonoid-derived components have been studied for their therapeutic properties.objectives: apigenin has shown remarkable antioxidant and anti-inflammatory features, sowe should have a reliable source of apigenin.methods: in this study, we used high-performance liquid chromatography method to comparethe amount of apigenin in flower, root, leaf, and stem of three varieties of osmos bipinnatus, i.e.,‘dazzler,’ ‘xanthos,’ ‘sensation pinkie’, and in transgenic root culture of c. bipinnatus ‘dazzler’.besides, the antioxidant activity of c. bipinnatus ‘dazzler’ transgenic root culture was evaluatedusing ferric reducing antioxidant power (frap) assay.results: dazzler variety flowers showed the highest recovery of apigenin with 0.799 mg/100mg dry weight (dw). however, the sensation pinkie variety leafs had the lowest recoverywith 0.089 mg/100mg. apigenin content in transformed roots (0.797 mg/100 mg dw) of c.bipinnatus ‘dazzler’ was significantly higher than non-transformed roots (0.42 mg/100 mgdw). the ethanolic extract of hairy root showed the frap value of 668.1 μm fe2+/mg that wascomparatively more than the wild root frap value (426.2 μm fe2+/mg).conclusion: in conclusion, the presence of apigenin in high amounts in hairy root culturesof c. bipinnatus ‘dazzler’ indicates its great potential for the future pharmaceutical industry.